Using a droplet generation chip designed by the BioMint Lab, we developed a control system to produce droplets with variable input diameters. Using Fluigent pressure pumps to drive the oil and aqueous phase flow rates, adjusting the aqueous pressure allows for variable droplet sizes.
Using Fluigent Pressure Pumps to drive to oil and aqueous flow rates, we optimize the rotation rate of the cell using a closed-loop control system. The cell-laden droplets are trapped in a microwell array and oil flows past the aqueous droplets. The microfludic chip design allows for high-throughput processing of the spinning cells and prepares them for imaging.
Once the 2D projections are collected from the rotating cell in step 2, a 3D reconstruction of the cell is produced. This 3D model can give insights to not only the topography, but different cell structures such as the nucleus by utilizing different flourescent dyes.
